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PDE6B antibody

Cat.#: 113666

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Product Information

  • Product Name
    PDE6B antibody
  • Documents
  • Description
    PDE6B Rabbit Polyclonal antibody. Positive WB detected in mouse eye tissue, A549 cells. Positive IP detected in Y79 cells. Observed molecular weight by Western-blot: 98 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CSNB3 antibody; GMP PDE beta antibody; PDE6B antibody; PDEB antibody; rd1 antibody; RP40 antibody
  • Immunogen
    Human PDE6B Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PDE6B recombinant protein (Accession Number: NM_001145291). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

  • Validations

    WB results of PDE6B antibody (Catalog No:113666) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

    WB results of PDE6B antibody (Catalog No:113666) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

    mouse eye tissue were subjected to SDS PAGE followed by western blot with Catalog No:113666(PDE6B antibody) at dilution of 1:1000

    mouse eye tissue were subjected to SDS PAGE followed by western blot with Catalog No:113666(PDE6B antibody) at dilution of 1:1000

    IP Result of anti-PDE6B (IP:Catalog No:113666, 4ug; Detection:Catalog No:113666 1:1000) with Y79 cells lysate 2000ug.

    IP Result of anti-PDE6B (IP:Catalog No:113666, 4ug; Detection:Catalog No:113666 1:1000) with Y79 cells lysate 2000ug.

  • Background
    PDE6 is a cGMP-specific PDE family and presents multicomponent enzyme complexes. The rod PDE6 enzyme is comprised of two catalytic subunits, PDE6A and PDE6B and it is expressed in human lungs(PMID:20979602). PDE6B(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta) is also named as PDEB and belongs to the cyclic nucleotide phosphodiesterase family. PDE6B also has a C-terminal CAAX motif for posttranslational processing involving lipidation, proteolysis, and carboxymethylation(PMID:8394243). Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40)(PMID:22334370) and congenital stationary night blindness autosomal dominant type 2 (CSNBAD2)(PMID:8075643).
  • References
    • Datta P, Allamargot C, Hudson JS. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proceedings of the National Academy of Sciences of the United States of America. 112(32):E4400-9. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"