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Product Name
PDCD2 antibody
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Description
PDCD2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, H7ES cells, mouse liver tissue. Positive IP detected in HeLa cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 37-39kd,45kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Zebrafish; other species not tested.
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Alternative names
PDCD2 antibody; programmed cell death 2 antibody; RP8 antibody; Zinc finger protein Rp 8 antibody; ZMYND7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDCD2 recombinant protein (Accession Number: NM_002598). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113763(PDCD2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113763(PDCD2 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-PDCD2 (IP:Catalog No:113763, 4ug; Detection:Catalog No:113763 1:500) with HeLa cells lysate 2800ug.
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Background
Programmed Cell Death 2 (PDCD2) is widely expressed in embryonic and adult tissues but with undefined function yet. Recent observation uncovered its role in hematopoietic stem cell differentiation during development. Catalog# 10725-1-AP is a rabbit polyclonal antibody raised against full-length PDCD2 of human origin.
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References
- Mu W, Munroe RJ, Barker AK, Schimenti JC. PDCD2 is essential for inner cell mass development and embryonic stem cell maintenance. Developmental biology. 347(2):279-88. 2010.
- Kramer J, Granier CJ, Davis S. PDCD2 controls hematopoietic stem cell differentiation during development. Stem cells and development. 22(1):58-72. 2013.
- Kokorina NA, Granier CJ, Zakharkin SO, Davis S, Rabson AB, Sabaawy HE. PDCD2 knockdown inhibits erythroid but not megakaryocytic lineage differentiation of human hematopoietic stem/progenitor cells. Experimental hematology. 40(12):1028-1042.e3. 2012.
- Liao H, Liu XJ, Blank JL. Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. Molecular & cellular proteomics : MCP. 10(11):M111.009183. 2011.
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