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Product Name
PAPSS1 antibody
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Description
PAPSS1 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, Jurkat cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, IHC, IP, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
APS kinase antibody; ATP sulfurylase antibody; ATPSK1 antibody; PAPS synthase 1 antibody; PAPSS antibody; PAPSS 1 antibody; PAPSS1 antibody; SAT Adenylyl sulfate kinase antibody; SK 1 antibody; SK1 antibody; Sulfate adenylate transferase antibody; Sulfurylase kinase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAPSS1 recombinant protein (Accession Number: NM_005443). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113578(PAPSS1 antibody) at dilution of 1:500
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:113578(PAPSS1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113578(PAPSS1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113578(PAPSS1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PAPSS1 (IP:Catalog No:113578, 4ug; Detection:Catalog No:113578 1:1000) with HepG2 cells lysate 1000ug.
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Background
PAPSS1 represents 3'-phosphoadenosine 5'-phosphosulfate synthase 1
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