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Product Name
PAK1 antibody
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Description
PAK1 Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in Jurkat cells, C6 cells, HeLa cells, K-562 cells, MCF7 cells, NIH/3T3 cells. Positive IF detected in C6 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 61-65 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Alpha PAK antibody; p21 activated kinase 1 antibody; p65 PAK antibody; PAK 1 antibody; PAK1 antibody; PAKalpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAK1 recombinant protein (Accession Number: NM_001376281). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113496(PAK1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:113496(PAK1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:113496(PAK1 Antibody) at dilution of 1:50
IP Result of anti-PAK1 (IP:Catalog No:113496, 4ug; Detection:Catalog No:113496 1:1000) with K-562 cells lysate 3200ug.
Immunofluorescent analysis of C6 cells using Catalog No:113496(PAK1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PAK1 represents p21 protein (Cdc42/Rac)-activated kinase 1
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