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Anti-Phospho-PAK1(Ser144)/2(Ser141)/3 (Ser154) Rabbit antibody

Cat.#: 167231

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Product Information

  • Product Name
    Anti-Phospho-PAK1(Ser144)/2(Ser141)/3 (Ser154) Rabbit antibody
  • Documents
  • Description
    Phospho-PAK1(Ser144)/2(Ser141)/3 (Ser154) Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    PAKalpha antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1
  • Clonality
    Polyclonal
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/2000-1/10000

    IHC: 1/20-1/100

    ICC/IF: 1/50

    FC: 1/20

    IP: 1/20

  • Validations

    Western blot detection of PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) in C6,3T3,Hela cell lysates using PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) Rabbit pAb(1:1000 diluted).Predicted band size:61kDa.Observed band size:61kDa.

    Western blot detection of PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) in C6,3T3,Hela cell lysates using PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) Rabbit pAb(1:1000 diluted).Predicted band size:61kDa.Observed band size:61kDa.

    Immunocytochemistry of PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154)(green) in Hela cells using PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) Rabbit pAb at dilution 1/50, and DAPI(blue)

    Immunocytochemistry of PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154)(green) in Hela cells using PAK1 (Phospho-Ser144)/PAK2 (Phospho-Ser141)/PAK3 (Phospho-Ser154) Rabbit pAb at dilution 1/50, and DAPI(blue)

  • Background
    Swiss-Prot Acc.O75914.Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"