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Product Name
PAICS antibody
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Description
PAICS Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human liver tissue, human testis tissue. Positive WB detected in Jurkat cells, HeLa cells, mouse brain tissue, mouse heart tissue, Raji cells. Observed molecular weight by Western-blot: 47kd
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Tested applications
ELISA, IHC, FC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADE2 antibody; ADE2H1 antibody; AIR carboxylase antibody; AIRC antibody; DKFZp781N1372 antibody; PAICS antibody; PAIS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAICS recombinant protein (Accession Number: NM_001079524). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113493(PAICS antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:113493(PAICS antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:113493(PAICS antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug PAICS antibody (Catalog No:113493, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PAICS is also named as ADE2(Multifunctional protein), AIRC, PAIS. It is an important bifunctional enzyme in de novo purine biosynthesis in vertebrate with both 5-aminoimidazole ribonucleotide carboxylase (AIRc) and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase (SAICARs) activities. It becomes an attractive target for rational anticancer drug design, since rapidly dividing cancer cells rely heavily on the purine de novo pathway for synthesis of adenine and guanine, whereas normal cells favor the salvage pathway(PMID:17224163).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"