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Product Name
PAF1 antibody
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Description
PAF1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells. Positive IP detected in HeLa cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 80 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
F23149_1 antibody; FLJ11123 antibody; hPAF1 antibody; PAF1 antibody; PD2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAF1 recombinant protein (Accession Number: NM_019088). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113484(PAF1 antibody) at dilution of 1:1000
IP Result of anti-PAF1 (IP:Catalog No:113484, 3ug; Detection:Catalog No:113484 1:1000) with HeLa cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113484(PAF1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113484(PAF1 antibody) at dilution of 1:100 (under 40x lens)
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Background
PAF1, also known as hPAF1 or PD2, is a PAF (RNA Polymerase II Associated Factor) complex subunit. Paf1 functions primarily to increase transcript elongation. The PAF complex also has a role in histone monoubiquitination. Paf1 is overexpressed in pancreatic cancer cells and has demonstrated potential oncogenic property. The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"