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Product Name
PAAF1 antibody
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Description
PAAF1 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HEK-293 cells, human brain tissue, Jurkat cells. Positive IHC detected in human testis tissue, human brain tissue, human kidney tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ11848 antibody; PAAF antibody; PAAF1 antibody; Protein G 16 antibody; Rpn14 antibody; WDR71 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAAF1 recombinant protein (Accession Number: NM_001267805). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113473(PAAF1 antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113473(PAAF1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113473(PAAF1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:113473(PAAF1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"