-
Product Name
P2RX4 antibody
- Documents
-
Description
P2RX4 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, human colon tissue, mouse brain tissue, SH-SY5Y cells. Positive IP detected in mouse brain tissue. Positive IF detected in HeLa cells. Positive IHC detected in human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 55-60kd,70-80kd
-
Tested applications
ELISA, WB, IHC, IF, FC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ATP receptor antibody; P2RX4 antibody; P2X purinOCeptor 4 antibody; P2X4 antibody; P2X4R antibody; Purinergic receptor antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of P2RX4 recombinant protein (Accession Number: NM_002560). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
Immunofluorescent analysis of HeLa cells using Catalog No:113542(P2RX4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug P2RX4 antibody (Catalog No:113542, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113542(P2RX4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113542(P2RX4 antibody) at dilution of 1:100 (under 40x lens)
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113542(P2RX4 antibody) at dilution of 1:300
IP Result of anti-P2RX4 (IP:Catalog No:113542, 4ug; Detection:Catalog No:113542 1:500) with mouse brain tissue lysate 4000ug.
-
Background
Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"