Product Name

P15RS antibody

Documents

Description

P15RS Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue, A431 cells, Jurkat cells, L02 cells, mouse liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 36 kDa

Tested applications

ELISA, WB, IF

Species reactivity

Human, Mouse; other species not tested.

Alternative names

RPRD1A antibody; P15RS antibody; p15INK4B related protein antibody; HsT3101 antibody; FLJ10656 antibody

Immunogen

Human P15RS Recombinant protein (His tag)

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of P15RS recombinant protein (Accession Number: NM_001303413). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IF: 1:20-1:200

Validations

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:113537(P15RS antibody) at dilution of 1:1000

Immunofluorescent analysis of HepG2 cells, using RPRD1A antibody Catalog No:113537 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Background

RPRD1A is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15(INK4b) and may have a role in cell cycle regulation. It contains a RAR domain that is involved in regulation of nuclear pre-mRNA, which suggests that P15RS may act as a nuclear regulation protein [PMID:12470661]. It also interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Besides, RPRD1A regulates cell cycle genes and attenuates WNT signaling [PMID: 20739273], and acts as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle [PMID: 22231121].

P15RS antibody

Product Information

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