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Product Name
p15 INK4b antibody
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Description
p15 INK4b Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 15 kDa
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Tested applications
ELISA, IHC, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDK4I antibody; CDKN2B antibody; INK4B antibody; MTS 2 antibody; MTS2 antibody; Multiple tumor suppressor 2 antibody; p14 INK4b antibody; P15 antibody; p15 INK4b antibody; p15INK4b antibody; TP15 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of p15 INK4b recombinant protein (Accession Number: NM_004936). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
WB result of p15 antibody with HEK293 (treated with TBHP for 0, 0.5, 1, 2, 3 and 4 hours).
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113535(CDKN2B antibody) at dilution of 1:100 (under 25x lens)
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113535(CDKN2B Antibody) at dilution of 1:600
IP Result of anti-CDKN2B (IP:Catalog No:113535, 4ug; Detection:Catalog No:113535 1:600) with HEK-293 cells lysate 2800ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:113535(CDKN2B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CDKN2B represents cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
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