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Osteopontin antibody

Cat.#: 113507

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Product Information

  • Product Name
    Osteopontin antibody
  • Documents
  • Description
    Osteopontin Rabbit Polyclonal antibody. Positive IHC detected in human stomach cancer tissue, human kidney tissue, human small intestine tissue. Positive WB detected in mouse kidney tissue, rat kidney tissue. Observed molecular weight by Western-blot: 66 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    BNSP antibody; Bone sialoprotein 1 antibody; BSPI antibody; ETA 1 antibody; Nephropontin antibody; OPN antibody; Osteopontin antibody; secreted phosphoprotein 1 antibody; SPP 1 antibody; SPP1 antibody; Urinary stone protein antibody; Uropontin antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Osteopontin recombinant protein (Accession Number: NM_000582). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:50-1:500

  • Validations

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113507(OPN, SPP1 Antibody) at dilution of 1:600

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113507(OPN, SPP1 Antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:113507(OPN, SPP1 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:113507(OPN, SPP1 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:113507(OPN, SPP1 Antibody) at dilution of 1:200 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:113507(OPN, SPP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Background
    Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"