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Product Name
Osteopontin antibody
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Description
Osteopontin Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, HEK-293 cells, HepG2 cells, Jurkat cells. Positive IF detected in HepG2 cells. Positive IHC detected in human stomach cancer tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BNSP antibody; Bone sialoprotein 1 antibody; BSPI antibody; ETA 1 antibody; Nephropontin antibody; OPN antibody; Osteopontin antibody; secreted phosphoprotein 1 antibody; SPP 1 antibody; SPP1 antibody; Urinary stone protein antibody; Uropontin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Osteopontin recombinant protein (Accession Number: NM_000582). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113506(OPN, SPP1 Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:113506(OPN, SPP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:113506(OPN, SPP1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:113506(OPN, SPP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.
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References
- Si J, Zhang J, Liu S. Characterization of a micro-roughened TiO2/ZrO2 coating: mechanical properties and HBMSC responses in vitro. Acta biochimica et biophysica Sinica. 46(7):572-81. 2014.
- Ying X, Zhao Y, Wang JL. Serum anti-osteopontin autoantibody as a novel diagnostic and prognostic biomarker in patients with hepatocellular carcinoma. Oncology reports. 32(4):1550-6. 2014.
- Yoon SP, Maeng YH, Hong R. Protective effects of epigallocatechin gallate (EGCG) on streptozotocin-induced diabetic nephropathy in mice. Acta histochemica. 116(8):1210-5. 2014.
- Si J, Dai J, Zhang J. Comparative investigation of human amniotic epithelial cells and mesenchymal stem cells for application in bone tissue engineering. Stem cells international. 2015:565732. 2015.
- Wang GZ, Cheng X, Zhou B. The chemokine CXCL13 in lung cancers associated with environmental polycyclic aromatic hydrocarbons pollution. eLife. 4:. 2015.
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