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Product Name
ORAI1 antibody
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Description
ORAI1 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive WB detected in Jurkat cells, A375 cells, MCF7 cells, mouse ovary tissue, mouse spleen tissue, PC-3 cells. Observed molecular weight by Western-blot: 45-47 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
CRACM1 antibody; FLJ14466 antibody; ORAI1 antibody; ORAI1 L1 antibody; ORAI1 L2 antibody; Orail L2 antibody; ORAT1 antibody; Protein orai 1 antibody; TMEM142A antibody; Transmembrane protein 142A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ORAI1 recombinant protein (Accession Number: NM_032790). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113416(ORAI1 antibody) at dilution of 1:800
Immunofluorescent analysis of HEK-293 cells using Catalog No:113416(ORAI1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Store-operated calcium channels (Socs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of Socs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
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References
- Braun A, Varga-Szabo D, Kleinschnitz C. Orai1 (CRACM1) is the platelet SOC channel and essential for pathological thrombus formation. Blood. 113(9):2056-63. 2009.
- Zhang ZY, Pan LJ, Zhang ZM. Functional interactions among STIM1, Orai1 and TRPC1 on the activation of SOCs in HL-7702 cells. Amino acids. 39(1):195-204. 2010.
- Eylenstein A, Gehring EM, Heise N. Stimulation of Ca2+-channel Orai1/STIM1 by serum- and glucocorticoid-inducible kinase 1 (SGK1). FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 25(6):2012-21. 2011.
- Eylenstein A, Schmidt S, Gu S. Transcription factor NF-κB regulates expression of pore-forming Ca2+ channel unit, Orai1, and its activator, STIM1, to control Ca2+ entry and affect cellular functions. The Journal of biological chemistry. 287(4):2719-30. 2012.
- Borst O, Schmidt EM, Münzer P. The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes. Blood. 119(1):251-61. 2012.
- Nurbaeva MK, Schmid E, Szteyn K. Enhanced Ca²⁺ entry and Na+/Ca²⁺ exchanger activity in dendritic cells from AMP-activated protein kinase-deficient mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 26(7):3049-58. 2012.
- Schmidt S, Schneider S, Yang W. TGFβ1 and SGK1-sensitive store-operated Ca2+ entry and Orai1 expression in endometrial Ishikawa cells. Molecular human reproduction. 20(2):139-47. 2014.
- Schmid E, Yan J, Nurbaeva MK. Decreased store operated Ca2+ entry in dendritic cells isolated from mice expressing PKB/SGK-resistant GSK3. PloS one. 9(2):e88637. 2014.
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