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Product Name
OPA3 antibody
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Description
OPA3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, human kidney tissue, Jurkat cells, mouse thymus tissue. Positive IP detected in HeLa cells. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 20 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ22187 antibody; FLJ25932 antibody; MGA3 antibody; OPA3 antibody; Optic atrophy 3 protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OPA3 recombinant protein (Accession Number: NM_025136). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113351(OPA3 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113351(OPA3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113351(OPA3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-OPA3 (IP:Catalog No:113351, 3ug; Detection:Catalog No:113351 1:400) with HeLa cells lysate 2440ug.
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Background
OPA3 represents optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
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References
- Owczarek-Lipska M, Plattet P, Zipperle L. A nonsense mutation in the optic atrophy 3 gene (OPA3) causes dilated cardiomyopathy in Red Holstein cattle. Genomics. 97(1):51-7. 2011.
- Powell KA, Davies JR, Taylor E, Wride MA, Votruba M. Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III. Investigative ophthalmology & visual science. 52(7):4369-80. 2011.
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