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Product Name
OGDHL antibody
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Description
OGDHL Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive WB detected in mouse kidney tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 115 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1290 antibody; OGDC E1 like antibody; OGDHL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OGDHL recombinant protein (Accession Number: XM_011539946). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113332(OGDHL antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:113332(OGDHL antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113332(OGDHL antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-OGDHL (IP:Catalog No:113332, 4ug; Detection:Catalog No:113332 1:500) with mouse brain tissue lysate 4000ug.
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Background
OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.
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References
- Sen T, Sen N, Noordhuis MG. OGDHL is a modifier of AKT-dependent signaling and NF-κB function. PloS one. 7(11):e48770. 2012.
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