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Product Name
NTHL1 antibody
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Description
NTHL1 Rabbit Polyclonal antibody. Positive IP detected in Y79 cells. Positive WB detected in Y79 cells. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NTH1 antibody; NTHL1 antibody; OCTS3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NTHL1 recombinant protein (Accession Number: NM_002528). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:113399(NTHL1 antibody) at dilution of 1:1000
IP Result of anti-NTHL1 (IP:Catalog No:113399, 4ug; Detection:Catalog No:113399 1:500) with Y79 cells lysate 2000ug.
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Background
NTHL1, also named as Endonuclease III-like protein 1 or ocTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"