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Product Name
NSDHL antibody
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Description
NSDHL Rabbit Polyclonal antibody. Positive IHC detected in human skin cancer tissue, human cervical cancer tissue, human colon cancer tissue, human colon tissue, human lung cancer tissue, human lung tissue, human ovary tissue, human ovary tumor tissue, human stomach cancer tissue, human stomach tissue. Positive WB detected in HeLa cells, human heart tissue, mouse brain tissue. Observed molecular weight by Western-blot: 42 kDa,40 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
H105E3 antibody; NSDHL antibody; Protein H105e3 antibody; SDR31E1 antibody; XAP104 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NSDHL recombinant protein (Accession Number: XM_011531178). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113286(NSDHL antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:113286(NSDHL antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:113286(NSDHL antibody) at dilution of 1:100 (under 40x lens)
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Background
NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130).
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References
- Sukhanova A, Gorin A, Serebriiskii IG. Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation. Cancer discovery. 3(1):96-111. 2013.
- Lewinska M, Juvan P, Perse M. Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of Cyp51 from cholesterol synthesis. PloS one. 9(11):e112787. 2014.
- Gabitova L, Restifo D, Gorin A. Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR. Cell reports. 12(11):1927-38. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"