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Product Name
NR0B2 Polyclonal Antibody
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Description
Polyclonal antibody to NR0B2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NR0B2 antibody; SHP antibody; SHP1 antibody; nuclear receptor subfamily 0 group B member 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human NR0B2 (NP_068804.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - NR0B2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NR0B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - NR0B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using NR0B2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - NR0B2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NR0B2 antibody . Blue: DAPI for nuclear staining.
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Background
Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"