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Product Name
NPTX2 antibody
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Description
NPTX2 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in mouse brain tissue, PC-3 cells, SH-SY5Y cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NARP antibody; Neuronal pentraxin 2 antibody; neuronal pentraxin II antibody; NPTX2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NPTX2 recombinant protein (Accession Number: NM_002523). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113225(NPTX2 antibody) at dilution of 1:100 (under 40x lens)
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113225(NPTX2 Antibody) at dilution of 1:600
IP Result of anti-NPTX2 (IP:Catalog No:113225, 4ug; Detection:Catalog No:113225 1:600) with mouse brain tissue lysate 4000ug.
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Background
Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is secreted protein involved in excitatory synapse formation. It also plays a role in clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in the Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)
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References
- Zhang L, Gao J, Li L, Li Z, Du Y, Gong Y. The neuronal pentraxin II gene (NPTX2) inhibit proliferation and invasion of pancreatic cancer cells in vitro. Molecular biology reports. 38(8):4903-11. 2011.
- Zhang L, Gao J, Li Z, Gong Y. Neuronal pentraxin II (NPTX2) is frequently down-regulated by promoter hypermethylation in pancreatic cancers. Digestive diseases and sciences. 57(10):2608-14. 2012.
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