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Product Name
NPHP5,IQCB1 antibody
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Description
NPHP5,IQCB1 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE1 cells.
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Tested applications
ELISA, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
IQ motif containing B1 antibody; IQCB1 antibody; KIAA0036 antibody; NephrOCystin 5 antibody; NPHP5 antibody; PIQ antibody; SLSN5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NPHP5,IQCB1 recombinant protein (Accession Number: NM_001023571). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
IF: 1:10-1:100
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Validations
IF Result of anti-NPHP5 (Catalog No:113215) show the base of cilia in human hTERT-RPE1 cells by Dr. Moshe Kim.
IF Result of anti-NPHP5 (Catalog No:113215,1:50) show 'focal adhesion-like' structures in human hTERT-RPE1 cells by Dr. Moshe Kim. 1% PFA fixation.
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Background
IQCB1, also known as NPHP5, is a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. It has a central coiled-coil region and two calmodulin-binding IQ domains. Localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells, IQCB1 is thought to play a role in ciliary function. Mutations in this gene result in Senior-Loken syndrome type 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.
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References
- Barbelanne M, Song J, Ahmadzai M, Tsang WY. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. Human molecular genetics. 22(12):2482-94. 2013.
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