NOTCH1 antibody

NOTCH1 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

NOTCH1 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, HeLa cells. Positive IHC detected in human kidney tissue, human lung tissue, human ovary tumor tissue, human spleen tissue, mouse brain tissue, mouse liver tissue. Observed molecular weight by Western-blot: 270-300 kDa

ELISA, WB, IP, IHC

Human,Mouse,Rat,Zebrafish; other species not tested.

hN1 antibody; Notch 1 antibody; NOTCH1 antibody; TAN1 antibody

Rabbit IgG

This antibody was obtained by immunization of Peptide (Accession Number: NM_017617). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

NOTCH1, also named as TAN1, belongs to the NOTCH family. NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. It may be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. NOTCH1 is involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV). Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and all the three cleaved NOTCH1 forms.

• Martin LJ, Katzenelson A, Koehler RC, Chang Q. The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells. PloS one. 8(11):e81105. 2013.
• Sun Q, Wang R, Wang Y, Luo J, Wang P, Cheng B. Notch1 is a potential therapeutic target for the treatment of human hepatitis B virus X protein-associated hepatocellular carcinoma. Oncology reports. 31(2):933-9. 2014.
• Annani-Akollor ME, Wang S, Fan J, Liu L, Padhiar AA, Zhang J. Downregulated protein O-fucosyl transferase 1 (Pofut1) expression exerts antiproliferative and antiadhesive effects on hepatocytes by inhibiting Notch signalling. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 68(6):785-90. 2014.