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Product Name
NOP2 antibody
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Description
NOP2 Rabbit Polyclonal antibody. Positive WB detected in A2780 cells, C6 cells, HeLa cells. Positive IF detected in HEK-293 cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 100-120 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
NOL1 antibody; NOP120 antibody; NOP2 antibody; NSUN1 antibody; Nucleolar protein 1 antibody; Nucleolar protein 2 homolog antibody; p120 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NOP2 recombinant protein (Accession Number: NM_001258308). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113303(NOP2 antibody) at dilution of 1:50 (under 10x lens)
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:113303(NOP2 Antibody) at dilution of 1:2000
Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:113303(NOP2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13.
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References
- Chamousset D, De Wever V, Moorhead GB. RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S ribosomal subunits. Molecular biology of the cell. 21(23):4212-26. 2010.
- Calkins AS, Iglehart JD, Lazaro JB. DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1. Nucleic acids research. 41(15):7378-86. 2013.
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