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Product Name
NNT antibody
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Description
NNT Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive WB detected in human adrenal gland tissue, human heart tissue, human liver tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 114 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NNT antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NNT recombinant protein (Accession Number: BC032370). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human adrenal gland tissue were subjected to SDS PAGE followed by western blot with Catalog No:113260(NNT antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:113260(NNT antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:113260(NNT antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-NNT (IP:Catalog No:113260, 4ug; Detection:Catalog No:113260 1:300) with HepG2 cells lysate 2800ug.
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Background
NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).
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References
- Fujisawa Y, Napoli E, Wong S. Impact of a novel homozygous mutation in nicotinamide nucleotide transhydrogenase on mitochondrial DNA integrity in a case of familial glucocorticoid deficiency. BBA clinical. 3:70-78. 2015.
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