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Product Name
NLRX1 antibody
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Description
NLRX1 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells, MCF7 cells, mouse heart tissue, mouse skeletal muscle tissue. Positive IHC detected in human heart tissue, human liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 100-110 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Caterpiller protein 11.3 antibody; CLR11.3 antibody; DLNB26 antibody; FLJ21478 antibody; NLR family member X1 antibody; NLRX1 antibody; NOD26 antibody; NOD5 antibody; NOD9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NLRX1 recombinant protein (Accession Number: BC013199). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113243(NLRX1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:113243(NLRX1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:113243(NLRX1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using NLRX1 antibody Catalog No:113243 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NLRX1 (IP:Catalog No:113243, 3ug; Detection:Catalog No:113243 1:800) with mouse heart tissue lysate 3520ug.
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Background
NLRX1 represents NLR family member X1
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References
- Soares F, Tattoli I, Wortzman ME, Arnoult D, Philpott DJ, Girardin SE. NLRX1 does not inhibit MAVS-dependent antiviral signalling. Innate immunity. 19(4):438-48. 2013.
- Soares F, Tattoli I, Rahman MA. The mitochondrial protein NLRX1 controls the balance between extrinsic and intrinsic apoptosis. The Journal of biological chemistry. 289(28):19317-30. 2014.
- Imbeault E, Mahvelati TM, Braun R, Gris P, Gris D. Nlrx1 regulates neuronal cell death. Molecular brain. 7:90. 2014.
- West AP, Khoury-Hanold W, Staron M. Mitochondrial DNA stress primes the antiviral innate immune response. Nature. 520(7548):553-7. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"