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Product Name
NKIAMRE antibody
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Description
NKIAMRE Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 52-60kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDKL3 antibody; cyclin dependent kinase like 3 antibody; NKIAMRE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NKIAMRE recombinant protein (Accession Number: NM_001113575). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113198(CDKL3 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113198(CDKL3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113198(CDKL3 antibody) at dilution of 1:50 (under 40x lens)
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Background
NKIAMRE is also named as CDKL3(Cyclin-dependent kinase-like 3) and belongs to the protein kinase superfamily. It restricts cell growth or maintaining differentiation and may have a role in regulating cell growth and/or diferentiation. This protein contains the MAPK-like TDY motif for threonine and tyrosine phosphorylation, as well as the putative cyclin-binding motif(PMID:10463609). It has 2 isoforms produced by alternative splicing with the molecular mass of 52 kDa and 68 kDa.
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