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Product Name
NKCC2 antibody
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Description
NKCC2 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive IF detected in mouse Embryonic kidney tissue. Positive WB detected in HEK-293 cells. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 55 kDa, 65-75 kDa
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Tested applications
ELISA, IF, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BSC1 antibody; NKCC2 antibody; SLC12A1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NKCC2 recombinant protein (Accession Number: BC040138). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
IF rsult of SLC12A1 antibody (Catalog No:113195, 1:900) with mouse Embryonic kidneys from E16.5 (fixed with 4% PFA at 4C for 30 min, cryosections 5% goat serum/PBST was used for blocking and antibody incubation). Red: anti-rabbit Alexa555, Blue: Hoechst.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:113195(SLC12A1 Antibody) at dilution of 1:200 (under 40x lens).
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:113195(SLC12A1 Antibody) at dilution of 1:200 (under 10x lens).
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113195(SLC12A1 Antibody) at dilution of 1:600
IP Result of anti-SLC12A1 (IP:Catalog No:113195, 4ug; Detection:Catalog No:113195 1:500) with mouse kidney tissue lysate 4000ug.
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Background
SLC12A1 represents solute carrier family 12 (sodium/potassium/chloride transporters), member 1
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