Product Name

NKCC1 antibody

Documents

Description

NKCC1 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in A549 cells, PC-3 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 170 kDa

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

Basolateral Na K Cl symporter antibody; BSC antibody; BSC2 antibody; NKCC1 antibody; SLC12A2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of NKCC1 recombinant protein (Accession Number: BC033003). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

Validations

A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:113194(SLC12A2 antibody) at dilution of 1:400

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113194(SLC12A2 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113194(SLC12A2 antibody) at dilution of 1:100 (under 40x lens)

IP Result of anti-SLC12A2 (IP:Catalog No:113194, 4ug; Detection:Catalog No:113194 1:500) with mouse brain tissue lysate 3600ug.

Background

SLC12A2 represents solute carrier family 12 (sodium/potassium/chloride transporters), member 2

References

Song P, Du Y, Song W. Bile deficiency induces changes in intestinal Cl(-) and HCO3 (-) secretions in mice. Acta physiologica (Oxford, England). 211(2):421-33. 2014.
Yang B, Rajput PS, Kumar U, Sastry BR. Regulation of GABA Equilibrium Potential by mGluRs in Rat Hippocampal CA1 Neurons. PloS one. 10(9):e0138215. 2015.

NKCC1 antibody

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