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Product Name
NF-L antibody
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Description
NF-L Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 65-68kd,140-160kd
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Tested applications
ELISA, WB, IP, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
68 kDa neurofilament protein antibody; CMT1F antibody; CMT2E antibody; FLJ53642 antibody; NEFL antibody; NF L antibody; NF68 antibody; NFL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NF-L recombinant protein (Accession Number: NM_006158). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113162(NEFL antibody) at dilution of 1:4000
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:113162(NEFL Antibody) at dilution of 1:200 (under 10x lens)
IP Result of anti-NEFL (IP:Catalog No:113162, 4ug; Detection:Catalog No:113162 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:113162(NEFL Antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:113162(NEFL Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NEFL, also named as NF68 and NFL, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd.This antibody is specific to NEFL. It has a very weak reaction to NEFM.
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References
- Yin F, Meng C, Lu R. Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injury by promoting axonal growth and anti-autophagy. Neural regeneration research. 9(18):1665-71. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"