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Product Name
NF-L antibody
- Documents
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Description
NF-L Mouse Monoclonal antibody. Positive IHC detected in human brain tissue. Positive WB detected in Human stomach, human brain tissue. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
68 kDa neurofilament protein antibody; NEFL antibody; Neurofilament L antibody; NF68 antibody; NFL antibody; NF-L antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of NF-L recombinant protein (Accession Number: NM_006158). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:107332(NEFL antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107332(NEFL Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107332(NEFL Antibody) at dilution of 1:200 (under 40x lens).
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Background
NEFL, also named as NF68 and NFL, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd.This antibody is specific to NEFL. It has a very weak reaction to NEFM.
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