NF-L antibody

Cat.#: 107332

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Product Information

  • Product Name
    NF-L antibody
  • Documents
  • Description
    NF-L Mouse Monoclonal antibody. Positive IHC detected in human brain tissue. Positive WB detected in Human stomach, human brain tissue. Observed molecular weight by Western-blot: 65 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    68 kDa neurofilament protein antibody; NEFL antibody; Neurofilament L antibody; NF68 antibody; NFL antibody; NF-L antibody
  • Immunogen
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of NF-L recombinant protein (Accession Number: NM_006158). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:107332(NEFL antibody) at dilution of 1:1000

    human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:107332(NEFL antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107332(NEFL Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107332(NEFL Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107332(NEFL Antibody) at dilution of 1:200 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107332(NEFL Antibody) at dilution of 1:200 (under 40x lens).

  • Background
    NEFL, also named as NF68 and NFL, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"