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Product Name
Neurotrophin 4 antibody
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Description
Neurotrophin 4 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in A431 cells, PC-3 cells, SH-SY5Y cells. Positive IHC detected in human testis tissue. Positive IF detected in PC-3 cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
neurotrophin 4 antibody; Neurotrophin 5 antibody; Neutrophic factor 4 antibody; NT 4 antibody; NT 4/5 antibody; NT 5 antibody; NT4 antibody; NT5 antibody; NTF4 antibody; NTF5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Neurotrophin 4 recombinant protein (Accession Number: NM_006179). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:113134(NTF4 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human testis using Catalog No:113134(NTF4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:113134(NTF4 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:113134(NTF4 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-NTF4 (IP:Catalog No:113134, 4ug; Detection:Catalog No:113134 1:300) with A431 cells lysate 2800ug.
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Background
NTF4 represents neurotrophin 4
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