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Product Name
NELFE Polyclonal Antibody
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Description
Polyclonal antibody to NELFE
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
NELFE antibody; D6S45 antibody; NELF-E antibody; RD antibody; RDBP antibody; RDP antibody; negative elongation factor E antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - NELFE Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NELFE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - NELFE Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NELFE antibody .
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Background
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA.; (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"