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Product Name
Anti-NELFe Rabbit antibody
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Description
NELFe Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
RD; RDP; RDBP; D6S45; NELF-E antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human NELFe
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/20
ICC/IF: 1/20
IP: 1/20
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Validations
Immunohistochemistry of NELFe in paraffin-embedded Human breast cancer tissue using NELFe Rabbit pAb at dilution 1/20
Western blot detection of NELFe in C6 cell lysates using NELFe Rabbit pAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.
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Background
Swiss-Prot Acc.P18615.Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"