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Product Name
NEK9 antibody
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Description
NEK9 Rabbit Polyclonal antibody. Positive IF detected in COS-7 cells.
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Tested applications
ELISA, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp434D0935 antibody; KIAA1995 antibody; Nek8 antibody; NEK9 antibody; NERCC antibody; NERCC1 antibody; Nercc1 kinase antibody; NimA related kinase 8 antibody; NimA related protein kinase 9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NEK9 recombinant protein (Accession Number: BC009336). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
IF: 1:20-1:200
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Validations
Immunofluorescent analysis of (10% Formaldehyde) fixed COS-7 cells using Catalog No:113107(NEK9 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is independently identified as a Nek6- and Ran GTPase- binding protein under a different name, Nercc1 (PMID:14660563). Nek9, together with the highly similar Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. Confocal microscopy identified that Nek9 was localized to the spindle poles at the metaphase stages and associated with the midbody at anaphase or telophase stage in both meiotic oocytes and the first mitotic embyros (PMID: 23159858). The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer (PMID:21454704).
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