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Product Name
Anti-NEK9 Rabbit antibody
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Description
NEK9 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NC; APUG; NERCC; LCCS10; NERCC1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human NEK9
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000
IHC: 1/20
ICC/IF: 1/50
FC: 1/20
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Validations
Western blot detection of NEK9 in Hela cell lysates using NEK9 Rabbit pAb(1:1000 diluted).Predicted band size:107kDa.Observed band size:107kDa.
Immunohistochemistry of NEK9 in paraffin-embedded Human colon cancer tissue using NEK9 Rabbit pAb at dilution 1/20
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Background
Swiss-Prot Acc.Q8TD19.Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"