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Product Name
NEDD1 antibody
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Description
NEDD1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, human brain tissue, SH-SY5Y cells. Positive IP detected in HeLa cells. Positive IF detected in HEK-293 cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 75 kDa; 62 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ35902 antibody; GCP WD antibody; NEDD 1 antibody; NEDD1 antibody; Protein NEDD1 antibody; TUBGCP7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NEDD1 recombinant protein (Accession Number: NM_152905). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113095(NEDD1 antibody) at dilution of 1:800
IP Result of anti-NEDD1 (IP:Catalog No:113095, 3ug; Detection:Catalog No:113095 1:800) with HeLa cells lysate 2150ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113095(NEDD1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113095(NEDD1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:113095(NEDD1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NEDD1 is required for mitosis progression. NEDD1 promotes the nucleation of microtubules from the spindle. This antibody works well in WB, IHC, IP and IF application.
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