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Product Name
NDUFB7 antibody
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Description
NDUFB7 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue, human cervical cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse ovary tissue, MCF7 cells, mouse brain tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B18 antibody; Cell adhesion protein SQM1 antibody; CI B18 antibody; Complex I B18 antibody; NDUFB7 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFB7 recombinant protein (Accession Number: NM_004146). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse ovary tissue were subjected to SDS PAGE followed by western blot with Catalog No:113076(NDUFB7 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:113076(NDUFB7 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113076(NDUFB7 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-NDUFB7 (IP:Catalog No:113076, 3ug; Detection:Catalog No:113076 1:500) with mouse brain tissue lysate 2640ug.
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Background
NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).
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References
- Meyer K, Buettner S, Ghezzi D, Zeviani M, Bano D, Nicotera P. Loss of apoptosis-inducing factor critically affects MIA40 function. Cell death & disease. 6:e1814. 2015.
- Chuang TW, Lee KM, Lou YC, Lu CC, Tarn WY. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. The Journal of biological chemistry. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"