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Product Name
NDUFB10 antibody
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Description
NDUFB10 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, human skeletal muscle tissue, mouse liver tissue. Positive IP detected in HepG2 cells. Positive IHC detected in human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CI PDSW antibody; Complex I PDSW antibody; NDUFB10 antibody; PDSW antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFB10 recombinant protein (Accession Number: NM_004548). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113070(NDUFB10 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:113070(NDUFB10 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:113070(NDUFB10 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-NDUFB10 (IP:Catalog No:113070, 4ug; Detection:Catalog No:113070 1:1000) with HepG2 cells lysate 1000ug.
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Background
NDUFB10(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10) is also named as PDSW and belongs to the complex I NDUFB10 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. The Complex I functions in the transfer of electrons from NADH to the respiratory chain.
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References
- Andrews B, Carroll J, Ding S, Fearnley IM, Walker JE. Assembly factors for the membrane arm of human complex I. Proceedings of the National Academy of Sciences of the United States of America. 110(47):18934-9. 2013.
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