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Product Name
NDUFA9 antibody
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Description
NDUFA9 Rabbit Polyclonal antibody. Positive WB detected in human skeletal muscle tissue. Positive IHC detected in human hepat℃irrhosis tissue. Observed molecular weight by Western-blot: 39kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CI 39kD antibody; Complex I 39kD antibody; NDUFA9 antibody; NDUFS2L antibody; SDR22E1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFA9 recombinant protein (Accession Number: NM_005002). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:113065(NDUFA9 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:113065(NDUFA9 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:113065(NDUFA9 antibody) at dilution of 1:50 (under 40x lens)
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Background
NDUFA9(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.
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References
- Shen SM, Yu Y, Wu ZX, Zheng Y, Chen GQ, Wang LS. Apoptosis-inducing factor is a target gene of C/EBPα and participates in adipocyte differentiation. FEBS letters. 585(14):2307-12. 2011.
- Zhang J, Wang C, Chen X. EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer. The EMBO journal. 34(23):2953-70. 2015.
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