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Product Name
NDUFA4L2 antibody
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Description
NDUFA4L2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, human brain tissue, human liver tissue. Positive IHC detected in human kidney tissue, human heart tissue, human liver tissue, human lung tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 10 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ26118 antibody; NDUFA4L2 antibody; NUOMS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFA4L2 recombinant protein (Accession Number: NM_001394960). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113060(NDUFA4L2 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113060(NDUFA4L2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113060(NDUFA4L2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using NDUFA4L2 antibody Catalog No:113060 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NDUFA4L2 (IP:Catalog No:113060, 4ug; Detection:Catalog No:113060 1:500) with HeLa cells lysate 1200ug.
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Background
NDUFA4L2(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.
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References
- Tello D, Balsa E, Acosta-Iborra B. Induction of the mitochondrial NDUFA4L2 protein by HIF-1α decreases oxygen consumption by inhibiting Complex I activity. Cell metabolism. 14(6):768-79. 2011.
- Castelblanco E, Gallel P, Ros S. Thyroid paraganglioma. Report of 3 cases and description of an immunohistochemical profile useful in the differential diagnosis with medullary thyroid carcinoma, based on complementary DNA array results. Human pathology. 43(7):1103-12. 2012.
- Heather LC, Cole MA, Tan JJ. Metabolic adaptation to chronic hypoxia in cardiac mitochondria. Basic research in cardiology. 107(3):268. 2012.
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