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Product Name
ND1 antibody
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Description
ND1 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HeLa cells, HepG2 cells. Positive IP detected in mouse liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue, human heart tissue. Observed molecular weight by Western-blot: 28-38 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
MT ND1 antibody; MTND1 antibody; NADH dehydrogenase subunit 1 antibody; NADH1 antibody; ND1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NC_012920). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113050(ND1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:113050(ND1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:113050(ND1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using Catalog No:113050(ND1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-ND1 (IP:Catalog No:113050, 3ug; Detection:Catalog No:113050 1:500) with mouse liver tissue lysate 4000ug.
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Background
ND1 belongs to the complex I subunit 1 family. ND1 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This antibody detects both isoforms of ND1, 318aa 34-36 kDa and 268aa, 28-30 kDa.
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References
- Zhang X, Zuo X, Yang B. MicroRNA directly enhances mitochondrial translation during muscle differentiation. Cell. 158(3):607-19. 2014.
- Zhang J, Wang C, Chen X. EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer. The EMBO journal. 34(23):2953-70. 2015.
- Chen H, Xu L, Yin L. iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells. Proteomics. 14(1):51-73. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"