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Product Name
NCL Polyclonal Antibody
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Description
Polyclonal antibody to NCL
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NCL antibody; C23 antibody; Nsr1 antibody; nucleolin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 461-710 of human NCL (NP_005372.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - NCL Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NCL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - NCL Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric using NCL antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - NCL Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using NCL antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - NCL Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using NCL antibody at dilution of 1:200 (40x lens).
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Background
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"