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Product Name
Anti-Phospho-Nucleolin (Thr84) Rabbit antibody
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Description
Phospho-Nucleolin (Thr84) Rabbit monoclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Alternative names
C23; Nsr1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Thr84 of human Nucleolin
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/5000
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Validations
Western blot detection of Phospho-Nucleolin (Thr84) in U251MG,HepG2,Hela cell lysates using Phospho-Nucleolin (Thr84) Rabbit mAb(1:500 diluted).Predicted band size:77kDa.Observed band size:100kDa.
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Background
Swiss-Prot Acc.P19338.Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"