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Product Name
NCL antibody
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Description
NCL Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue, human cervical cancer tissue, human prostate hyperplasia tissue. Positive IP detected in HeLa cells. Positive WB detected in HEK-293 cells, rat brain tissue. Observed molecular weight by Western-blot: 100-110 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
C23 antibody; FLJ45706 antibody; NCL antibody; nucleolin antibody; Protein C23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NCL recombinant protein (Accession Number: BC006494). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113040(NCL antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113040(NCL antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-NCL (IP:Catalog No:113040, 4ug; Detection:Catalog No:113040 1:1000) with HeLa cells lysate 3200ug.
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Background
Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation.
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References
- Yang X, Zou P, Yao J. Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma. Journal of proteome research. 9(3):1402-15. 2010.
- Du R, Long J, Yao J. Subcellular quantitative proteomics reveals multiple pathway cross-talk that coordinates specific signaling and transcriptional regulation for the early host response to LPS. Journal of proteome research. 9(4):1805-21. 2010.
- Xing Y, Shi Z. Nucleocapsid protein VP15 of White spot syndrome virus colocalizes with the nucleolar proteins nucleolin and fibrillarin. Canadian journal of microbiology. 57(9):759-64. 2011.
- Tang S, Bao H, Zhang Y, Yao J, Yang P, Chen X. 14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage. PloS one. 8(3):e55268. 2013.
- Tang S, Bai C, Yang P, Chen X. 14-3-3ε boosts bleomycin-induced DNA damage response by inhibiting the drug-resistant activity of MVP. Journal of proteome research. 12(6):2511-24. 2013.
- Bao H, Song P, Liu Q. Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers. Proteomics. Clinical applications. 3(6):705-19. 2009.
- Haeusler AR, Donnelly CJ, Periz G. C9orf72 nucleotide repeat structures initiate molecular cascades of disease. Nature. 507(7491):195-200. 2014.
- Wu DM, Zhang P, Liu RY. Phosphorylation and changes in the distribution of nucleolin promote tumor metastasis via the PI3K/Akt pathway in colorectal carcinoma. FEBS letters. 588(10):1921-9. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"