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Product Name
NCF4 Polyclonal Antibody
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Description
Polyclonal antibody to NCF4
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NCF4 antibody; CGD3 antibody; NCF antibody; P40PHOX antibody; SH3PXD4 antibody; neutrophil cytosol factor 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NCF4 (NP_038202.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - NCF4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NCF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - NCF4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using NCF4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NCF4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human thyroid cancer using NCF4 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NCF4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using NCF4 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - NCF4 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NCF4 antibody . Blue: DAPI for nuclear staining.
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Background
Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"