-
Product Name
NCF4 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to NCF4
-
Tested applications
WB, IHC, FC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
NCF4 antibody; CGD3 antibody; NCF antibody; P40PHOX antibody; SH3PXD4 antibody; neutrophil cytosol factor 4 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide of human NCF4
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, pH7.3.
-
Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
FC 1:20 - 1:50 -
Validations
Western blot - NCF4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NCF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.
-
Background
Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"