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Product Name
NCALD antibody
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Description
NCALD Mouse Monoclonal antibody. Positive WB detected in Human brain, human brain tissue. Positive IHC detected in human cerebellum tissue, human testis tissue. Observed molecular weight by Western-blot: 22kD
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
NCALD antibody; neurOCalcin delta antibody; neurOCalcin δ antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of NCALD recombinant protein (Accession Number: XM_017013902). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107323(NCALD antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cerebellum using Catalog No:107323(NCALD antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:107323(NCALD Antibody) at dilution of 1:50
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Background
NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.
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