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Product Name
NAPRT1 antibody
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Description
NAPRT1 Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human small intestine tissue. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells. Observed molecular weight by Western-blot: 51 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FHA HIT interacting protein antibody; FHIP antibody; NAPRT1 antibody; NAPRTase antibody; PP3856 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of NAPRT1 recombinant protein (Accession Number: NM_001363146). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107320(NAPRT1 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:107320(NAPRT1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:107320(NAPRT1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:107320(NAPRT1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"