Cart 2

NALP3 antibody

Cat.#: 113019

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    NALP3 antibody
  • Documents
  • Description
    NALP3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human bladder tissue, human breast cancer tissue, human oesophagus tissue, human tonsillitis tissue. Positive WB detected in K-562 cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 108-110 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    AGTAVPRL antibody; AII antibody; AII/AVP antibody; AVP antibody; C1orf7 antibody; Caterpiller protein 1.1 antibody; CIAS1 antibody; CLR1.1 antibody; Cryopyrin antibody; FCAS antibody; FCU antibody; FLJ95925 antibody; MWS antibody; NALP3 antibody; NLRP3 antibody; PYPAF1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_001127461). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113019(NALP3 antibody) at dilution of 1:500

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113019(NALP3 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human bladder using Catalog No:113019(NALP3 antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human bladder using Catalog No:113019(NALP3 antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human bladder using Catalog No:113019(NALP3 antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemical of paraffin-embedded human bladder using Catalog No:113019(NALP3 antibody) at dilution of 1:200 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using NLRP3 antibody Catalog No:113019 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using NLRP3 antibody Catalog No:113019 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID). The antibody recognizes the C-term of NALP3.
  • References
    • Wang CP, Zhang LZ, Li GC. Mulberroside A protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion. Journal of neuroscience research. 92(7):944-54. 2014.
    • Yang F, Wang Z, Wei X. NLRP3 deficiency ameliorates neurovascular damage in experimental ischemic stroke. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 34(4):660-7. 2014.
    • Gao P, Meng XF, Su H. Thioredoxin-interacting protein mediates NALP3 inflammasome activation in podocytes during diabetic nephropathy. Biochimica et biophysica acta. 1843(11):2448-60. 2014.
    • Peng K, Liu L, Wei D. P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation. International journal of molecular medicine. 35(5):1179-88. 2015.
    • E Q, Tang M, Zhang X. Protection of seven dibenzocyclooctadiene lignans from Schisandra chinensis against serum and glucose deprivation injury in SH-SY5Y cells. Cell biology international. 39(12):1418-24. 2015.
    • Wu Q, Liu MC, Yang J, Wang JF, Zhu YH. Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation. Applied and environmental microbiology. 82(4):1173-82. 2015.
    • Gao Z, Han Y, Hu Y. Targeting HO-1 by Epigallocatechin-3-Gallate Reduces Contrast-Induced Renal Injury via Anti-Oxidative Stress and Anti-Inflammation Pathways. PloS one. 11(2):e0149032. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"