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Product Name
MYSM1-Specific antibody
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Description
MYSM1-Specific Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A549 cells, HEK-293 cells, HepG2 cells. Positive IHC detected in human spleen tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 81 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
2A DUB antibody; 2ADUB antibody; DKFZp779J1554 antibody; DKFZp779J1721 antibody; KIAA1915 antibody; MYSM1 antibody; RP4 592A1.1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001085487). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113002(MYSM1-Specific antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human spleen using Catalog No:113002(MYSM1-Specific antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-MYSM1-Specific (IP:Catalog No:113002, 4ug; Detection:Catalog No:113002 1:300) with HeLa cells lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:113002(MYSM1-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.
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